CULTIVATION OF HUMAN INDUCED PLURIPOTENT STEM CELLS WITH CONTROLLED AGGREGATE SIZE AND GEOMETRICAL ARRANGEMENT BY INVERTING MICROWELL ARRAY CHIP

This paper reports a novel cell culture chip, namely “inverting microwell array chip”, for cultivation of human induced pluripotent stem cells with controlled microenvironment. Our “inverting microwell array chip” comprises a lower hydrogel microwell array and an upper polystyrene (PS) culture surface. We demonstrate the formation of uniform human induced pluripotent stem cell aggregates in the microwell array, and after inversion, a culture with controlled aggregate size and geometrical arrangement on the PS surface.