Establishment in Vitro of a Human Neurogenic Sarcoma

A human neurogenic sarcoma was established in vitro and maintained in continuous growth for over 5 years. The cells exhibit morphological characteristics of neurosarcoma at the light and electron microscope level. The karyotype is aneuploid (near-triploid to near-tetraploid) and presents marker chromosomes. The doubling time is 63 hr. The generation time is 58 hr, and the length of the different phases of the cell cycle are G1, 27.5 hr, S, 22 hr, and G2, 8.5 hr. An estimated 90% of the cells are in the proliferative pool. This cell line, designated T2, is suggested as a working in vitro model for human neurosarcoma. Preliminary data with the use of immunofluorescent techniques indicate that the cells may possess a common sarcoma tumor antigen.