Drug Delivery using Nanoparticle along with ssDNA

Recently nanoparticles have been developed for the photo acoustic imaging, delivery of genes and laser induced photo thermal therapy. In this study, we have developed ssDNA conjugated nanoparticles as the carrier for simultaneous DNA and anti-cancer nucleoside delivery. The ssDNA nanoparticle complex presented higher capacity in carrying anti-cancer drug compounds than the original nanoparticles. The hydrodynamic size of the nanoparticles increased from 25 to 35 nm with an increase in the negative surface charge from -9.58 to 21.66 mV after ssDNA conjugation and drug loading. A positive association between environmental pH and drug release was observed in PBS and Tris/HCl, which implied their potential use in the controlled localized drug release in the lower GI tract. The Tryphan Blue, Ethidium Bromide assay revealed dose dependent cytotoxicity to oral and lung cancer cell line than free compounds. These results suggest the potential use of this new ssDNA nanoparticles complex as the environmental controlled anticancer nanocapsule, especially suitable for oral and lung cancer chemotherapy.