[Change of plasma endothelin-1 concentrations in photodynamic induced rat anterior ischemic optic neuropathy model and drug modulation].

Objective To investigate changes of plasma endothelin-1 (ET-1) concentration in photodynamic induced rat anterior ischemic optic neuropathy model (rAION) and evaluate the effects of compound anisodine hydrobromide ( CA ). Methods Eighty-five Sprague-Dawley (SD) male rats were randomly divided into a blank control group of 10 rats and a model group of 75 rats. rAION model was established in the model group by photodynamic induction. The model group was divided into a rA1ON simple group of 25 rats, a CA intervention group of 25 rats, and a normal saline ( NS ) control group of 25 rats. Beginning from the day that the rAION model was established, temporal subcutaneous injections (once daily for 3 days) of CA and NS were performed in the CA and the NS groups, respectively. The plasma ET-1 concentrations were detected by radioimmunoassay and analyzed at 1, 3, 5, 7 and 14 days.Results The means plasma ET-1 concentration of rAION simple group is ( 114.9 ± 17.6) ng/L, higher than that of the control group (69. 4 ± 9. 1 ) ng/L (t = 14. 92, P ＜0. 01 ). In the rAION model group, the plasma ET-1 concentrations 1 to 5 days after the model was established were higher than that of 7 to 14 days.During observational periods, on the 1st, 5th, 7th and 14th day, there was no significant difference between the CA and NS groups ( t =0. 58, 2. 07, 0. 81 and 0. 93, P ＞0. 05 ), but on the 3rd day the level of plasma ET-1 concentration in the CA group was significantly lower than that of the NS group ( t= 4. 72, P ＜ 0. 05 ).Conclusions Increase of plasma ET-1 concentrations may play an important role in the pathogenesis of photodynamic induced rAION model. CA can decrease the plasma ET-1 concentrations in rAION rats. Key words: Rats;  Optic neuropathy, ischemic;  Endothelin-1;  Scopo lamine derivatives; Photochemotherapy