Free-radical degradation of high-molar-mass hyaluronan induced by ascorbate plus cupric ions: testing of stobadine and its two derivatives in function as antioxidants.

Stobadine·2HCl and its two hydrophilic derivatives SM1dM9dM10·2HCl and SME1i- ProC2·HCl were tested in the function of antioxidants on hyaluronan (HA) degradation induced by the Weissberger oxidative system (ascorbate plus Cu(II)). As a primary method, rotational vis- cometry was applied, where the substance tested was added before or 1 h after the initiation of HA degradation. The most effective scavengers ofOH and peroxy-type radicals were recorded to be stobadine·2HCl and SME1i-ProC2·HCl, respectively. The most effective scavenger, determined by applying the ABTS assay, was stobadine·2HCl.