Automatic attenuation correction technique for fluorescent analysis of the photosensitizer concentration in biological tissues

At present, spectroscopic methods of intraoperative determination of photosensitizer distribution in organs and tissues are advanced, since they provide a quick and accurate result with minimal impact on a biological object. However, the interpretation of fluorescence spectra is problematic since fluorescence from tissues is significantly affected by absorption and scattering at both the excitation and emission wavelengths. In this regard, we studied the influence of optical properties on the intensity of the fluorescence signal, and we proposed an algorithm for analyzing the concentration of the photosensitizer by the fluorescence signal, leveling this effect.