High hydrostatic pressure activates transcription factors involved in Saccharomyces cerevisiae stress tolerance.
2012
A number of transcriptional control elements are activated when Saccharomyces cerevisiae cells are submitted
to various stress conditions, including high hydrostatic pressure (HHP). Exposure of Saccharomyces cerevisiae cells to
HHP results in global transcriptional reprogramming, similar to that observed under other industrial stresses, such as temperature,
ethanol and oxidative stresses. Moreover, treatment with a mild hydrostatic pressure renders yeast cells multistress
tolerant. In order to identify transcriptional factors involved in coordinating response to high hydrostatic pressure,
we performed a time series microarray expression analysis on a wild S. cerevisiae strain exposed to 50 MPa for 30 min
followed by recovery at atmospheric pressure (0.1 MPa) for 5, 10 and 15 min. We identified transcription factors and corresponding
DNA and RNA motifs targeted in response to hydrostatic pressure. Moreover, we observed that different motif
elements are present in the promoters of induced or repressed genes during HHP treatment. Overall, as we have already
published, mild HHP treatment to wild yeast cells provides multiple protection mechanisms, and this study suggests that
the TFs and motifs identified as responding to HHP may be informative for a wide range of other biotechnological and industrial
applications, such as fermentation, that may utilize HHP treatment.
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