Growth of test microorganisms used in determining antibiotic activity on synthetic nutrient media

: The growth of gram-negative sporulating bacteria and yeast-like fungi used in a microbilogical assay of antibiotic activity was studied on solid synthetic media of simple composition. Their reproduction with the microbial growth of different density was shown to be possible on media containing available and strictly standardized components, such as salts, glucose and disubstituted sodium phosphate. The cultures tested were not similar by their growth requirements.