A Tetracycline Repressor-based Mammalian Two-hybrid System to Detect Protein-protein Interactions in vivo

Abstract A mammalian two-hybrid system (termed as trM2H) was developed to detect protein–protein interactions in vivo , based on the reconstitution of the functions the of tetracycline repressor (TetR). The system is sensitive enough to detect protein–protein interactions with K d up to 55 μM in mammalian cells, and the system can be regulated by small molecules. This system can be used as an efficient genetic selection system to map protein–protein interactions in mammalian cells.