Parentage Analysis of Freedom Rootstock

The rootstock Freedom is the offspring of two open-pollinated parents (1613-59 and Dog Ridge 5), sources of root-knot nematode resistance. As phylloxera has increased in importance in vineyards, the risk of Vitis vinifera -derived phylloxera susceptibility from the unknown pollen donors is a concern. To clarify the pedigree of Freedom, genetic profiles of 31 potential progenitors were analyzed with 30 microsatellite markers. We confirm the reported parent (1613-59) and grandparent (1613C) of Freedom and identify 3306C ( V. riparia x V. rupestris ) as the pollen parent of 1613-59. We were unable to identify the pollen parent of Dog Ridge 5.