Chromosomal localization of NDPK gene in Zhikong scallop Chlamys farreri using BAC-FISH

Zhikong scallop(Chlamys farreri) is one of the most economically important aquaculture species in China.Using the technology of BAC-FISH,we localized the BAC clones containing NDPK gene in a pair of ho-mologous chromosomes.This result proved the reliability of the contig assembling of the C.farreri physical map,at the same time,the localization of NDPK gene in chromosome provided further support for research of structures,functions and the application of the gene.This research is the practice of localizing genes of low copies.The result will provide reference to further research and characterization of C.farreri chromosomes,and will facilitate the integration of the physical map,linkage map and cytogenetics map of this species.