Image cytometric DNA analysis in transitional cell carcinoma of the bladder

Background. The current study was initiated to investigate measurable objective and reproducible characteristics that might have prognostic significance in bladder cancer, Methods. Tumor samples from 91 patients with primary transitional cell carcinoma (TCC) of the urinary bladder were studied by DNA image cytometry and cytogenetic analysis. Image cytometry is a more sensitive method of determining ploidy than flow cytometry, especially in tumors with a low number of aneuploid cells. Results. There was a significant difference in survival between DNA image cytometry-determined diploid and nondiploid cases. The presence of nuclei with a high DNA content indicated poor prognosis. The 2C deviation index (2CDI) also was an indicator of survival