Screening of Chinese herbal flavone ingredient prescriptions of anti-virus in vitro

In order to screen ideal flavone prescriptions of antivirus in vitro,nine total flavone ingredients prescriptions were formed based on the four total flavones of EF,BSRF,WDFF,and SF,which showed better antivirus activities described in previous experiment.Every prescription was diluted into five series concentrations from its maximal safe concentration,then,added into CEF monolayer with three ways.MTT colorimetric assay was used to detect the effects of the nine prescriptions anti-NDV in CEF.The results indicated that all of the prescriptions displayed antivirus abilities in some degree when treated by the above three treatments.Evaluated by D570 values and the highest virus inhibition ratio,three prescriptions of epimedium total flavones-baikal skullcap root total flavones(EF-BSRF),epimedium total flavones-wild dendranthema flower total flavones-baikal skullcap root total flavones(EF-WDFF-BSRE),epimedium total flavones-sanchi total flavones-wild dendranthema flower total flavones-baikal skullcap root total flavones(EF-SF-WDFF-BSRF) showed better antivirus activities.