Molecular and electrophysiological characterization of a allelic variant of the rat α6 GABAA receptor subunit
1992
Abstract A 1.45 kb DNA sequence encoding the rat α6 GABA A receptor subunit (nucleotides 33–1483) was clonedfrom a Sprague-Dawley rat brain cDNA library by PCR amplification. Dideoxy sequencing of two individual clones revealed that the nucleotide sequence differed at only one basepair (T 480 → G) from that published previously. This difference altered the deduced amino acid sequence, producing a conservative amino acid substitution (His 121 → Gln). A Gln residue is present at the same location in the bovine α6 subunit. Restriction endonuclease analysis of the total PCR product demonstrated that this variant of the rat α6 subunit was the only allele found in this particular rat brain library; the original allele was not present. These results were further verified by RNAse protection assays performed with RNA isolated from individual rat cerebella. α6, β1, and γ2S subunits were transiently expressed in L929 cells for electrophysiological analysis. Whole-cell recordings obtained from the cells demonstrated that GABA A receptor channels with the expected GABA and benzodiazepine pharmacology were produced. Excised outside out single channel recordings from the same cells revealed that GABA elicited brief duration openings to a 33 pS main conductance level and to at least one smaller (approximately 21 pS) subconductance level. Thus this allelic variant of rat α6 subunit could assemble with other subunits to form a functional GABA A receptor channel with similar properties to the original allelic form.
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