Validation of the procedure for the determination of aflatoxin B1 in animal liver using immunoaffinity columns and liquid chromatography with postcolumn derivatisation and fluorescence detection

Abstract The validation of the procedure for the determination of aflatoxin B 1 in animal liver (pig, chicken, turkey, beef, calf) was performed. The limit of detection (LOD) and limit of quantification (LOQ) were 2 ng/kg and 7.8 ng/kg, respectively. The repeatability of measurements, represented by the standard deviation (RSD r ) was 7.5%, 7.1%, and 4.8% at the contamination levels of 0.025 μg/kg, 0.050 μg/kg, and 0.075 μg/kg, respectively. The relative standard deviation for the within-laboratory reproducibility (RSD R ) was 18% at the level of 0.025 μg/kg and 22% at the levels of 0.050 μg/kg and 0.075 μg/kg. The measurement uncertainties at the same contamination levels were ±0.007 μg/kg, ±0.016 μg/kg, and ±0.023 μg/kg, respectively. The mean recovery was 72.8%, the decision limit (CCα) 0.063 μg/kg and the detection capability (CCβ) 0.080 μg/kg. The results indicate that the procedure is suitable for the determination of aflatoxin B 1 in animal liver and can be implemented for the routine analysis.