Effects of Benner Pury on endothelial progenitor cells apoptosis and oxidative stress in patients with hypertension

Objective To investigate the effection of Benner Pury on endothelial progenitor cells apoptosis and oxidative stress in vitro hypertension. Methods The patients who were diagnosed as hypertension grade 1, according to the standard ″Guidelines for Prevention and Treatment of Hypertension″. The control group were healthy subjects matched with study group in age and gender. Mononuclear cells were isolated by density gradient centrifugation, and fluorescence chemical staining in acLDL-DiI, FITC-lectin, observed under confocal laser scanning microscope, staining positive cells were considered as the differentiation of endothelial progenitor cell(EPC). EPC cultivated for 5 days were used for study. The cells isolated from each hypertensive were divided into five copies in parallel, and according to the different stimulation, the cells were divided into five groups: hypertension group, different concentrations of Benner Pury (1 μmol/L, 10 μmol/L, 100 μmol/L) intervention in hypertension group, and Benner Pury (10 μmol/L)+ stromal cell derived factor-1(SDF-1)/its receptor chemokine 4 (CXCR4) antagonist AMD 3100 pretreatment group. EPC cultivated for 24 h under the different stimulation were used for study EPC apoptosis detection: Annexin-V/PI method; oxidative stress detection: the activity of superoxide dismutase (SOD), xanthine-oxidation method, the content of Malonaldehyde (MDA) malondialdehyde thiobarbituric acid method. Results (1) Compared with the control group, peripheral blood EPS apoptosis rate was obviously increased in hypertension group, oxidative stress was significantly increased too (tearly=6.6334, tlate=3.812; both P<0.01). (2) Detection of the apoptosis and oxidative stress level display, different concentrations of Benner Pury 1 μmol/L, 10 μmol/L, 100 μmol/L intervention in vitro hypertensive patients EPC for 24 h, with the concentrations of Benner Pury gradually increased, the apoptosis rate of EPC was reduced (Fearly=17.39, Flate=51.65; both P<0.05), oxidative stress response also come down in a concentration dependent manner (FSOD=14.56, FMDA=7.859; both P<0.05). Compared with 10 μmol/L pretreatment group, 10 μmol/L+ group AMD 3100, EPC apoptosis rate increased (tearly=3.551, tlate=5.333, both P<0.05), oxidative stress response raised up (tSOD=1.931, tMDA=0.6407, both P<0.05). Conclusions Benner Pury significantly improved the apoptosis of EPC and oxidative stress response in vitro hypertension, and the function may be through the SDF-1/CXCR4 axis. Key words: Hypertension; Endothelial progenitor cells; Apoptosis; Oxidative stress; Antagonist AMD 3100