Marsupial genetics studied in tammar, parma and black-striped wallabies: mapping, conservation and evolution

"August, 1994"%%%%%%%%%%%%1 : Literature review  – 2. Aims  – 3. Breeding within and between populations of tammar wallabies  – 4. Genetic variation in the tammar: implications for conservation  – 5. Linkage mapping through recombination in the tammar wallaby  – 6. Levels of genetic variation at MHC class II loci in the tammar wallaby  – 7. Conservation genetics of the parma wallaby  – 8. Discussion.%%%%This thesis reports work on the breeding and genetic typing of several species of wallaby. The work was initiated with two main aims in mind. The first was to develop a marsupial species for use as an organism for genetic work, in the first instance genetic mapping. The second was to investigate genetic variability within and between populations of these wallabies, with consideration of conservation priorities and taxonomic relationships. A third aim, to examine MHC variability was added as data on this group of loci became available during the course of the investigation.  – Development of an efficient system for generating a linkage map of a marsupial genome through recombination has been difficult in the past largely due to lack of detectable genetic variability within the study species. Two different systems were examined for their potential usefulness. One was an interspecies cross between the parma wallaby and its nearest relative the black-striped wallaby. The other was a cross between two different island populations of tammar wallabies, one from Kangaroo Island (KI) in South Australia and the other from Garden Island (GI) in Western Australia.  – Despite reports by others of hybridisation between parmas and black-stripes, no Fl hybrids were produced in either direction during the period of this research. Hence, this interspecies cross was not useful for linkage mapping.  – The between sub-species cross was however highly successful. Fl hybrids were produced between KI and GI tammars and both males and females were backcrossed to the parental strains. Approximately 200 testcross progeny were generated for mapping studies over a three year period. Large quantities of DNA and several different tissues have been stored from each progeny allowing numerous investigations to be carried out in the future.  – KI and GI tammar wallabies were typed for 54 genetic markers. These data were used to compare the levels of heterozygosity within and between the two populations. This was done with a view to examining the effects of isolation on small island populations of macropodids. KI is one of Australia's largest offshore islands (450,000 ha) whereas GI is approximately 430 times smaller (1054 ha). Surprisingly, the level of genetic variation in GI tammars is not significantly different to that seen in the much larger KI population.  – Of the 54 markers used 19 showed large gene frequency or fixed differences between the two populations. Of these, 15 were used to type backcross progeny for the mapping study. Seven of these belonged to three linkage groups, two autosomal and one X-linked. Identification of large gene…