EFFECTS OF DRUGS AGAINST ANTIOXIDANT AND CYTOTOXIC (HEp 2 CELL LINE) ACTIVITY COMPOUNDS FROM MARINE ANIMALS CONUS AMADIS VENOM (GMELIN, J.F, 1791)

Objective: The present study was carried out to explore the hemolytic, antioxidant and cytotoxic activity of conotoxins extracted from the venom of C. amadis . Methods: The crude conotoxin was extracted and tested for cytotoxic, antioxidant activity and cancer cell lines. The cytotoxic activity was studied by brine shrimp cells and i n-vitro antioxidant activity were determined by DPPH, Superoxide anion radical scavenging assay and hydrogen peroxide scavenging from active fraction of conopeptides. The active fractions were tested in HEp-2 cancer cell lines at different concentration. Results: The different dose tested viz 100μg/mL -1 , 50μg/mL -1 , 25μg/mL -1 , 12.5μg/mL -1 , 6.25μg/mL -1 , and 3.2μg/mL -1 cytotoxicity on HEp-2 cell was observed at the 25μg/mL. HEp-2 cell displayed dose dependent decreased in viability detected as early as 48 hrs. The purified conotoxin was showed remarkable cytotoxicity against HEp-2 cell in a dose depend manner. The isolated conotoxin that produced maximum effect at 100μg/mL -1 and the half inhibitory concentrations (IC 50 ) was 25μg/mL -1 in the concentration of 45.6±0.8%. Conclusion: The present study revealed that cono-peptides from C. amadis have been used as an accessible source of natural antioxidants as well as anticancer after trial with animal and pre-clinical studies. Keywords: Gastropod, Conus amadis, Venom gland, Antioxidant and HEp-2 Cell line and MTT assay