[A fluorescent-probe detecting CYP2C9 * 3 DNA].

The mismatched CYP2C9*3 DNA was detected by a exciplex fluorescent-probe system.The exciplex fluorescent-probe model system comprises of two 12-mer oligonucleotides fluorescent-probes,complementary to neighbouring sites of a 24(47)-mer DNA target and plasmid target,each equipped with moieties able to form an exciplex on correct,contiguous hybridization.Very similar results were obtained between the 24-mer target and the 47-mer target when WT and MT systems were detected by the exciplex fluorescent-probe.Exciplex bands at 505 nm were found for both 24(47)-mer WT and 24(47)-mer MT-targets at 5 ℃,but were not distinctive enough to distinguish 24(47)-mer WT-target and 24(47)-mer MT-target.However the experiments were carried out at Tm,the exciplex band disappeared almost completely for 24(47)-mer MT-target system like control system,and there was still a strong exciplex band for the 24(47)-mer WT target system.Exciplex peaks at 505 nm were seen for the WT circular plasmids system,but not for MT circular plasmids.Therefore,mismatches of CYP2C9*3 DNA can be effectively detected by this exciplex construct,giving potential for single nucleotide polymorphism detection.