Enhancing the effect of catechins with green tea flavonol and polysaccharides on preventing lipid absorption and accumulation

Abstract The hypothesis of the current study was that flavonol and polysaccharides obtained during catechins extraction from green tea may enhance the effect of catechins on preventing pancreatic lipase activity, free glycerol absorption and accumulation in adipocytes co-cultured with Caco-2 cell after in vitro digestion. Catechins rich extract from green tea (GCT: 10 mg), GCT formulated with polysaccharides (GTP: 1 mg) and flavonol extract (GFV: 0.1 mg) strongly inhibited lipase activity during digestion while increasing lipolysis in adipocytes. Lipid accumulation in adipocytes was significantly reduced in GCT (10 mg) + GTP (1 mg) + GFV (1 mg) (100–87.27%) compared to control (p