Monoclonal antibody for inhibiting formation of bacterial biofilm

The invention belongs to the field of biotechnology and relates to a monoclonal antibody for inhibiting the formation of a bacterial biofilm and a B-cell epitope directed by the monoclonal antibody. The monoclonal antibody is prepared by taking a protein truncation body AapBrpt 1.5 in the B structural domain of a Staphylococcus epidermidis aggregation Aap protein as an antigen and immunizing a Bal b/c mouse; and the monoclonal antibody can identify a specific epitope in the Aap protein and inhibit the function of the Aap protein, so as to play a role in inhibiting the formation of a Staphylococcus epidermidis biofilm. The monoclonal antibody provided by the invention can be used for preparing medicines for curing biofilm-related diseases caused by Gram-positive cocci; and the B-cell antigenic epitope polypeptide directed by the monoclonal antibody can be used for preparing a vaccine resisting to the formation of a Gram-positive cocci biofilm.