Cytotoxic lanostane-type triterpenoids from the fruiting bodies of Ganoderma lucidum and their structure–activity relationships

// Shaodan Chen 1, 2, * , Xiangmin Li 1, 2, * , Tianqiao Yong 1, 2, * , Zhanggen Wang 3 , Jiyan Su 1, 2 , Chunwei Jiao 2 , Yizhen Xie 1, 2 , Burton B. Yang 4, 5 1 State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Institute of Microbiology, Guangzhou, China 2 Yuewei Edible Fungi Technology Co. Ltd., Guangzhou, China 3 College of Chinese Materia Medica, Guangzhou University of Traditional Chinese Medicine, Guangzhou, China 4 Sunnybrook Research Institute, Sunnybrook Health Sciences Centre, Toronto, Canada 5 Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Canada * These authors have contributed equally to this work Correspondence to: Yizhen Xie, email: 13622216490@126.com Burton B. Yang, email: byang@sri.utoronto.ca Keywords: Ganoderma lucidum, lanostane-type triterpenoids, cytotoxicity, 3D-QSAR Received: July 25, 2016      Accepted: December 01, 2016      Published: December 28, 2016 ABSTRACT We conducted a study of Ganoderma lucidum metabolites and isolated 35 lanostane-type triterpenoids, including 5 new ganoderols ( 1 - 5 ). By spectroscopy, we compared the structures of these compounds with known related compounds in this group. All of the isolated compounds were assayed for their effect against the human breast carcinoma cell line MDA-MB-231 and hepatocellular carcinoma cell line HepG2. Corresponding three-dimensional quantitative structure–activity relationship (3D-QSAR) models were built and analyzed using Discovery Studio. These results provide further evidence for anti-cancer constituents within Ganoderma lucidum , and may provide a theoretical foundation for designing novel therapeutic compounds.