Character of Eukaryotic Expression of Chicken B-LB Gene

B-LB gene encodes the β chain of chicken MHC(major histocompatibility complex) calssⅡmolecule, which plays an important role in antigens presentation.The full-length B-LB gene with a length of 792 bp was amplified by RT-PCR method from chicken lymphocytes.Then this segment was transfected into COS7 and P815 cells with Lipofectin reagent respectively.B-LB gene could be expressed on palsma membrane of COS7 cells;A cell strain of stable expression of B-LB gene,P815-B-LB,was obtained by adding G418(0.6 mg/mL).After the cell passed for 10 times the specific B-LB segment could be amplified by RT-PCR.All the results showed that a cell strain of P815(B-LB) with stable expression of B-LB was successfully established,which proved a foundation for further researching the effects of MHCⅡclass molecule β chain in immune response.