Visualization of carboxylesterase 2 with a near-infrared two-photon fluorescent probe and its potential evaluation of anticancer drug effects in orthotopic colon carcinoma mice model

We establish a near-infrared two-photon fluorescent probe for the detection of CES2 with high selectivity and sensitivity. This probe exhibits low cytotoxicity and superior tissue penetration ability for evaluating real-time activity of CES2 in living cells, in cancer tissues, and in colon carcinoma mice model.