Development and Validation of Spectrophotometric Method for Simultaneous Estimation of Trimetazidine Hydrochloride and Metoprolol Succinate in Pharmaceutical Dosage Form

A simple, rapid, precise and accurate spectrophotometric method has been developed for determination of Trimetazidine Hydrochloride and Metoprolol Succinate by absorbance ratio method in combined dosage form. The absorbance ratio method is based on measurement of absorbance at 249.5 nm (isoabsorptive point) and 274 nm (λ max of Metoprolol Succinate) as two wavelengths selected for quantification of Trimetazidine Hydrochloride and Metoprolol Succinate. Method obeyed Beer's law in the concentration range of 40-200 µg/ml for Trimetazidine Hydrochloride and 54-270 µg/ml for Metoprolol Succinate. The proposed methods were validated and can be used for analysis of combined dosage tablet formulation containing Trimetazidine Hydrochloride and Metoprolol Succinate.