Identification of HLA Alleles with Low or No Cell Surface Expression in the Czech Population

The presence of the A*24020102L allele is implicated in one donor from the CBMD who serolog- ically was typed as A2; B44, B55; Cw1, Cw7. The DRB4*01030102N allele was identified in one healthy donor and in one patient with MDS during routine HLA class II DNA typing. The DRB4*01030102N allele was identified in the patientis father, who had CML, and was associated with the HLA-A3-B7-Cw7-DRB1*0701-DQB1*0303 haplotype, which is common for European popula- tions. In order to avoid mistyping, both techniques, serology and molecular biology must be used for HLA typing, especially for cases where just one antigen appeared to be present using serological methods.