The action of instilled physostigmine on the acetylcholine content of the bovine retina

: Kymographic measurements of acetylcholine (ACh) in bovine retinal tissue carried out on guinea-pig ileum do not indicate a significant elevated concentration of ACh in the eye instilled with physostingmine salicylate (1% aqueous solution, 10 applications of three drops at intervals of 3 minutes). The eyes were enucleated one hour after the last application.