Ectomycorrhizas of two species of Tuber (clade Puberulum) in the Mexican subtropical cloud forest

Tuber species are hypogeous mycorrhizal symbionts distributed almost worldwide and associated with several tree families. In this study, we recorded the occurrence of two Tuber species (clade Puberulum) in the Acatlan Volcano (Veracruz, Eastern Mexico). They were detected by the means of the internal transcribed spacer (ITS) of nuclear ribosomal DNA sequences obtained from ectomycorrhizal samples collected at the site. The Tuber ectomycorrhizas were related symbiotically with Alnus acuminata and Fagus grandifolia var. mexicana. This is the first report of Tuber associated with these trees. The anatomical and morphological characterization of the ectomycorrhizae are presented along with illustrations.