The establishment of serologically detecting method on core type of Escherichia coli

Objective To prepare the core type monospecific antisera of Escherichia coli (E.coli). To establish serological detecting method for core types of E.coli. To detect the distribution of lipopolysacchride core types in pathogenic E.coli. Methods ELISA indirect method, we detected lipopolysacchride core types of E.coli using absorbed lipopolysacchride core type monospecific antisera in E.coli. Results (1) The respective core type antisera were made as a monospecific one by repeated absorption. Lipopolysacchride core types of E.coli by absorbed lipopolysacchride core type monospecific antisera is same as that by the monoclone antibody; (2) The results proved that the components being recognized and bound by absorbed core type monospecific antisera was neither nuclei acid and protein nor lipopolysaccharide lipid A and inner core KDO heptopyranose, an but outer core hexose antigen composing E.coli lipopolysaccharide core types; (3) Lipopolysaccharide core types of 27 strains of different serotype pathogenic E.coli from 4 classes had been detected. The results showed: 15 strains (55.5%) expressed R1 core type, and 10 (37.1%) and 2 (7.4%) strains expressed R3 and K12 core types respectively. None of the 27 strains of E.coli expressed R2 and R4 core type. Conclusion The respective core type antisera were made higher monospecific by repeated absorption, and it can be used in detecting E.coli lipopolysaccharide core types.