Broadband Hyperspectral Stimulated Raman Scattering Microscopy with a Parabolic Fiber Amplifier Source

In most hyperspectral SRS imaging experiments, the SRS spectral range is limited by the total bandwidth of the excitation laser to ~300 cm−1 and spectral resolution of ~20 cm−1. Here we present a novel approach for broadband hsSRS microscopy based on parabolic fiber amplification to provide linearly chirped broadened Stokes pulses. This novel hsSRS instrument provides >600 cm--−1 spectral coverage and ~10 cm−1 spectral resolution.