Analysis of potassium iodate reduction in tissue homogenates using high performance liquid chromatography-inductively coupled plasma-mass spectrometry.

Abstract Potassium iodate (KIO 3 ) and potassium iodide (KI) are the major salt iodization agents used worldwide. Unlike iodide (I − ), iodate (IO 3 − ) should be reduced to I − before it can be effectively used by the thyroid. In this study, we developed a new method for analyzing IO 3 − and I − in tissue homogenates using high performance liquid chromatography coupled to inductively coupled plasma mass spectrometry (HPLC–ICP-MS). We further applied the method to demonstrate the KIO 3 reduction process by tissues in vitro . The effects of KIO 3 on the total antioxidative activity (TAA) and reduced nicotinamide adenine dinucleotide phosphate (NADPH) were also investigated here. Finally, we found that IO 3 − can be reduced to I − by tissue homogenates and IO 3 − irreversibly decreases the antioxidant capability of tissues. Our studies suggest that KIO 3 might have a big effect on the redox balance of tissue and would further result in oxidative stress of organisms.