Depth-encoded spectral domain phase microscopy for simultaneous multisite nanoscale optical measurements of nerve activation
2009
Spectral Domain Phase Microscopy (SDPM) is a recent extension of Spectral Domain Optical Coherence Tomography
(SDOCT) that exploits the extraordinary phase stability of spectrometer-based systems with common-path geometry to
resolve sub-wavelength displacements within a sample volume. This technique has been implemented for high resolution
axial displacement and velocity measurements in biological samples, but since axial displacement information is
acquired serially, has been unable to measure fast temporal dynamics in extended samples. Depth-Encoded SDPM
(DESDPM) uses multiple sample arms with unevenly spaced common path reference reflectors to multiplex independent
SDPM signals from separate lateral positions on a sample simultaneously using a single interferometer, thus limiting the
time required to detect unique optical events to the integration time of the detector. The minimum measured sample
displacements determined from the standard deviation of the detected phase as a function of time two ideal reflectors
were 407 and 730 pm. Heat-induced expansion in a microscope slide was measured at two sites simultaneously. A 51 ms
delay in 50% rise time of the surface displacement was measured. Further application of this technique to biological
samples could yield insight into temporal dynamics of activation signals.
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