Effect of carbohydrate-feeding strategy on the production, physiochemical changes, anti-inflammation activities of polysaccharides of Poria cocos.

The aim of this research was to physiochemically characterize the structure and study the pharmaceutical benefits of the polysaccharide (PS) produced by Poria cocos using two selected carbohydrates (sucrose, and potato dextrose broth) in the in vitro culture system. A direct dosage effect was shown as sucrose- or PDB-based medium on the PS yield of Paragalago cocos. Very low-molecular-weight PS (<1 kDa) were largely synthesized by sucrose and PDB feeding. Sucrose-feeding mycelia of P. cocos results in a direct dosage effect in the fructose component in the PS. Sucrose and PDB feeding increased the glucose content but decreased the galactose content of PS. This study examined the anti-inflammatory activities of PS in lipopolysaccharide (LPS)-induced RAW264.7 macrophages. At 100 μg/mL and 50 μg/mL, PS from 10 g/L PDB- treatment, denoted as PDB 10, pretreatment showed maximal inhibition of TNF-α and IL-6 release, respectively. Mechanically, PDB10 attenuated IκB from degradation in LPS-induced macrophages, and down-regulated LPS-induced phosphorylation of ERK/AKT/p-38. PDB10 showed dose-dependent inhibition of the LPS induced TGFRII signaling pathways.