THE SELECTION AND CONTENT DETERMINATION OF AN ANTI-MIGRAINE EFFECTIVE FRACTION SEPARATED FROM TIANMA GOUTENG DECOCTION BY MACROPOROUS RESIN

The objective of the present study was to obtain an effective part of Tianma Gouteng decoction (TMGTD) on anti-migraine and to establish a RP-HPLC method for the content determination of Jasminoidin and Baicalin in the part. For this study, TMGTD extract was prepared by decoction with water and 30%, 60% and 90% part of TMGTD were made by D-101 macroporous resin. Migraine rat model induced by subcutaneous injection of Nitroglycerine was applied to evaluate the anti-migraine effect of the samples. RP-HPLC coupled with liquid-solid extraction method was applied for the content determination of Jasminoidin and Baicalin. Results: 60% fraction was the most effective part of TMGTD on anti-migraine, with significant effect on reducing the frequency of head scratching and regulating the abnormal content of 5-HT, DA, NE and E in serum and brain tissue of migraine rats. The calibration curve of Jasminoidin and Baicalin was linear (r＞0.999) over the range of 0.0153 to 0.183 μg and 0.334 to 4.013 μg respectively, and the average recovery of each component was from 96.1% to 100.1% and 97.6% to 102.7% respectively. The content of Jasminoidin and Baicalin in 60% fraction was 1.03% and 29.6%, which was significantly higher than that in the fraction before purification process optimization. Conclusions: This was the first report on anti-migraine effective part selection and main components content determination of TMGTD. The result may provide a meaningful basis for the clinical application of TMGTD on anti migraine headache and for the new product development of TMGTD effective part.