Modification of monolithic stationary phase using human serum albumin as chiral separation

Enantiomers can have different pharmacological effects. Therefore, it is necessary to be able to separate pure enantiomers that have a beneficial biological activity. The purpose of this study was to modify a monolith column in stationary phase with Human Serum Albumin (HSA) for use in separating chiral compounds by HPLC. In this work, poly(glycidyl methacrylate-co-ethylene glycol dimethacrylate) (poly(GMA-co-EDMA)) and poly(glycidyl methacrylate-co-trimethylolpropane trimethacrylate) (poly(GMA-co-TRIM)) columns were in-situ co-polymerized inside a silicosteel tubing. A total monomer ratio (% T) and crosslinking agent (% C) 40:25 and 28:12 were applied for the preparation of poly(GMA-co-EDMA) and poly(GMA-co-TRIM), respectively. The porogen used was 1-propanol, 1,4-butanediol, and water in a ratio of 7:4:1 (v/v) and AIBN as an initiator (1% of the total monomer). The column was polymerized at 60 °C for 12 h, then immobilized with various concentrations of Human Serum Albumin (HSA). The monolithic poly(GMA-co-TRIM) column modified with HSA 1 mg/ml was successfully applied to separation of the enantiomers of citronellal using an acetonitrile and water (50:50, v/v) as the mobile phase at 0.05 ml/min. The permeability of this optimized monolithic column was 0.0018 Darcy.Enantiomers can have different pharmacological effects. Therefore, it is necessary to be able to separate pure enantiomers that have a beneficial biological activity. The purpose of this study was to modify a monolith column in stationary phase with Human Serum Albumin (HSA) for use in separating chiral compounds by HPLC. In this work, poly(glycidyl methacrylate-co-ethylene glycol dimethacrylate) (poly(GMA-co-EDMA)) and poly(glycidyl methacrylate-co-trimethylolpropane trimethacrylate) (poly(GMA-co-TRIM)) columns were in-situ co-polymerized inside a silicosteel tubing. A total monomer ratio (% T) and crosslinking agent (% C) 40:25 and 28:12 were applied for the preparation of poly(GMA-co-EDMA) and poly(GMA-co-TRIM), respectively. The porogen used was 1-propanol, 1,4-butanediol, and water in a ratio of 7:4:1 (v/v) and AIBN as an initiator (1% of the total monomer). The column was polymerized at 60 °C for 12 h, then immobilized with various concentrations of Human Serum Albumin (HSA). The monolithic poly(GMA...