Apical extrusion of intracanal biofilm using ProTaper Gold, WaveOne Gold,Twisted File Adaptive, OneShape New Generation and K3XF

Objective To evaluate the bacterial extrusion during instrumentation with different nickel titanium (NiTi) engine-driven instruments. Methods Ninety extracted single-canal human mandibular incisor teeth were inoculated with Enterococcus faecalis to obtain biofilm formation and were randomly divided to 6 groups (n=15). One group served as the control and was not instrumented; the other groups were prepared with ProTaper Gold (PTG; Dentsply Maillefer, Ballaigues, Switzerland), WaveOne Gold (WOG; Dentsply Maillefer), Twisted File Adaptive (TFA; SybronEndo, Orange, CA, USA), One Shape New Generation (OSNG; MicroMega, Besancon, France), and K3XF (SybronEndo) instruments. Bacteria extruded beyond the apical foramen were quantified in colony-forming units per milliliter. The number of colony-forming units in the remaining biofilm was determined for each sample. Data were analyzed using the one-way analysis of variance (ANOVA) and Tukey post-hoc tests. Results All NiTi instruments resulted in different quantities of bacterial extrusion. The TFA group caused most bacterial extrusion (P 0.05). Conclusion PTG and WOG are preferable system in terms of successful endodontic treatments. The amount of bacterial extrusion is associated with the metallurgy and design of the instrument used.