Evaluation of MRSA selective medium in detection of methicillin-resistant staphylococcus aureus from hands and nasal vestibular of the medical staffs

Objective To analysis the detection of methicillin-resistant Staphylococcus aureus(MRSA) from the hands and nasal vestibular of doctors, nurses and attendants, and compare the detection effect for MRSA of two kinds of test methods. Methods A total of 678 hand specimens and 179 nasopharyngeal vestibular specimens of medical staffs were treated with two kinds of test methods for identification of MRSA. Firstly, we collected specimens with blood agar plates and then identified the strains by automatic microorganism instrument after screening. At the same time, the drug sensitivity tests with cefoxitin(30μg/) and oxacillin(1μg/) were performed by K-B disk diffusion method for identification of MRSA; meanwhile, all the specimens were directly inoculated on MRSA selective chromogenic medium to identify MRSA. At last, the effects of two test methods for identification of MRSA were compared. Results A total of 5 strains of MRSA and 44 strains of methicillin sensitive Staphylococcus aureus(MSSA)were detected in the hand specimens, also 3 strains of MRSA and 22 strains of MSSA were detected in the nasopharyngeal vestibular specimens by using the traditional method; 6 strains of MRSA and 43 strains of MSSA were detected in the hand specimens, 3strains of MRSA and 22 strains of MSSA were detected in the nasopharyngeal vestibular specimens by using the rapid method.Conclusion Comparing with the traditional detection method, the total coincidence rate of the rapid method for MRSA identification was 95.9% in the hand specimens and 100% in the nasopharyngeal vestibular specimens. The detection results of two test methods for MRSA and MSSA showed no significant difference(P0.05). Because of the simple operation and high specificity of the rapid method, it is suitable for popularization in clinical laboratory for identification of MRSA.