Dynamic Changes in the Expression of Myogenic Regulatory Factors in Mdx Mice after Micro-Dystrophin Gene Modified Autologous Bone Mesenchymal Stems Cells Transplantation

Objective To study the mechanism of differentiation of genetically modified autologous mesenchymal stem cells into functional muscle cells in transplanted mdx mice.Methods mMSCs were infected with retrovirus containing micro-dystrophin and transplanted into mdx mice through tail vein.After transplantation,the expression of micro-dystrophin was determined using immunofluorescence.The expression of MyoD was also determined.Results After transplantation,micro-dystrophin positive fibers were increased in the gastrocnemius.By immunofluorescence,RT-PCR and Western blotting methods,the expression of MyoD was also found to be time-dependently increased.Conclusion The genetically modified autologous MSCs was found to differentiate into micro-dystrophin positive cells in transplanted mdx mice,and myogenic regulatory factors appeared to play a key pole in this process.