HPLC-MS/MS determination of residue level of Kryptofix 2.2.2 in ~(18)F-FDG

In this work,we focused at establishing an efficient and rapid method of high-performance liquid chromatography-tandem mass spectrometry(HPLC/MS/MS) for quantitative analysis of Kryptofix 2.2.2 as 18F-FDG tracer.K-222 and internal standard were chromatographically separated on an ultimate XB-C18 column(Φ4.6 mm ×150 mm,3 μm) with a mobile phase containing 40 mmol/L ammonium acetate buffer and acetonitrile(50:50) at a flow rate of 0.85 mL.min–1.The electronic spray ion tandem mass spectrum with the positive mode and multitude reaction monitor(MRM) were used to detect K-222 in [18F]FDG..The linear range of calibration curves was 0.5–120 ng.min–1for K-222,with the intrabatch and innerbatch variations of 9.4%.The recovery rate ranged from 101.3% to 106.6%.The study indicated that the present method was rapid(less than 4 min),sensitive,efficient,and reliable,being suitable for detecting K-222 in18F-FDG.