Effects of TSP-2 Antibody,Specific for the Extracellular Domain of Murine TLR2,on C48/80-Induced Degranulation of P815 Cells

Objective Aims to examine the effect of antibody(TSP-2),specific for the epitope on murine TLR2 extracellular domain,on C48/80-induced degranulation of P815 cells.Methods The expression of mTLR2 in P815 cells was determined by RT-PCR and immunohistochemical methods.The effect of antibody on degranulation was determined by histochemical methods and confocal microscopy.Results Expression of mRNA and membrane TLR2 was observed in untreated P815 cells.Antibody TSP-2 was found to suppress C48/80-induced morphological changes and Ca2+ efflux in P815 cells.Conclusion TSP-2 is a specific antibody for the TLR2.This antibody can protect P815 cells from degranulation induced by C48/80.