Identification of foliumn pyrrosiae from different habitats and species by HPLC fingerprint

Objective:To establish HPLC fingerprint of Folium Pyrrosiae for identification.Methods:The HPLC method was developed with Diamonsil C18 column(250 mm×4.6 mm,5 μm),and a mixture liquid of acetonitrile-0.8% acetic acid solution as mobile phase in a gradient elution.HPLC fingerprints of 44 samples were analyzed by similarity,cluster and principal component analysis.Results:The HPLC fingerprint common pattern of Pyrrosia petiolosa,Pyrrosia lingua and common pattern of Pyrrosia sheareri were set up separately.Samples from different species were classified based on the result of cluster and principal component analysis.Fingerprints of Pyrrosia sheareri and Pyrrosia lingua have high degree of similarity,but were different from Pyrrosia petiolosa,while Pyrrosia calvata and Pyrrosia assimlis were classified as adulterants with their dissimilar fingerprints.Conclusion:The method is stable and reliable with a good reproducibility and provides a reference standard for identifying Folium Pyrrosiae from different habitats and species.