Characteristics of promoter AtRD29a from Arabidopsis thaliana during its expression in tobacco.

GUS gene controlled by promoter AtRD29a of chilling induction type was introduced into tobacco,with dyeing of GUS active tissue,to analyse the characteristics of promoter AtRD29a from Arabidopsis thaliana during its expression in tobacco.The result showed that the exogenous gene had been successfully integrated into Tobacco genome.Same as in Arabidopsis thaliana,the promoter AtRD29a had no specificity of tissue expression in tobacco and it belonged to chilling induction type,which had no expression under ordinary temperature of 25 ℃ and a efficient and stable expression around 4 ℃,with a critical temperature of 10 ℃ for induction.