Differences in Cystic Fibrosis-Associated Burkholderia spp. Bacteria Metabolomes after Exposure to the Antibiotic Trimethoprim.
2020
The Burkholderia cepacia complex is a group of closely related
bacterial species with large genomes that infect immunocompromised
individuals and those living with cystic fibrosis. Some of these species
are found more frequently and cause more severe disease than others,
yet metabolomic differences between these have not been described.
Furthermore, our understanding of how these species respond to antibiotics
is limited. We investigated the metabolomics differences between three
most prevalent Burkholderia spp. associated with
cystic fibrosis: B. cenocepacia, B. multivorans, and B. dolosa in the presence and absence of the
antibiotic trimethoprim. Using a combination of supervised and unsupervised
metabolomics data visualization and analysis tools, we describe the
overall differences between strains of the same species and between
species. Specifically, we report, for the first time, the role of
the pyomelanin pathway in the metabolism of trimethoprim. We also
report differences in the detection of known secondary metabolites
such as fragin, ornibactin, and N-acylhomoserine
lactones and their analogs in closely related strains. Furthermore,
we highlight the potential for the discovery of new secondary metabolites
in clinical strains of Burkholderia spp. The metabolomics
differences described in this study highlight the personalized nature
of closely related Burkholderia strains.
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