The Establishment of a Wound Model Induced by Ultrapulse $CO_2$ Laser

Background: Wound model is important for the development of dressing materials in dermatologic practice, Most of the wound healing models are not standardized, individualized ones for each special situation. Objectives: The purpose of our study is to establish a standardized wound model for evaluating the efficiency of wound healing of dressing material and the effect of epidermal growth factor(EGF) on artificially induced wounds. Methods: Using ultrapulse laser and rats, we calibrates the most effective level of energy, frequency, and pulse duration for a wound model of upper dermis. Then, we evaluated the effect of various dressing materials such as vaseline gauze, collagen sponge, collagen sponge containing EGF and hydrogel on wound healing. Results: We obtained relatively even and uniformed wound models of upper dermal level with the setting of ultrapulse 1aser; continuous mode, 800watt, frequency of 100㎐, and pulse duration of 0.3㎳. Wounds applied with collagen sponge containing EGF showed relatively accelerated wound healing, but wounds with hydrogel or collagen sponge only showed delayed healing of wounds. Conclusion: Ultra laser could make a wound model of uniform depth level and could be used as a tool for making standardized wound models.