Study on factors involved in formation of in vitro potato seedlings and microtuber

The effects of sterilization methods and various combinations of BA and 2, 4-D in different concentrations on induction of adventitious buds, of light condition on microtuber formation, and of method of forcing on rate of bud sprouting in potato were studied. The results indicated that the sterilization using 70% ethanol 30 sec, 0.1% HgCl_(2) 1 min improved survival rate of the stem meristem; the combination of 0.4 mg/L BA and 2 mg/L 2, 4-D enhanced dventitious bud induction; low supplemental light increased microtuber yield; thorned microtuber that experienced soaking in a solution of 0.5 mg/L 2, 4-D and GA_(3) for 8 hours gave the best rate of bud sprouting.