Plant Regeneration from Leaf Mesophyll Protoplasts of Lycopersicon glandulosum

Mesophyll protoplasts isolated from Lycopersicon glandulosum were cultured in the modified 8E liquid medium. These protoplasts were divided after 5 days of culture. When the fresh liquid medium was added to the culture on the 7th day, the protoplasts continued to divide for 14 days to form colonies. These colonies were transferred to the modified 8E agar medium, then transferred again to MS agar medium containing 1.0mg/l zeatin to induce shoots. The induced shoots finally developed into intact plants on MS hormone-free agar medium.