Characterization and optimization of production of bacterial cellulose from strain CGMCC 17276 based on whole-genome analysis

Abstract Bacterial cellulose (BC) has received considerable attention as an environment-friendly, biodegradable nanomaterial. In this study, the strain Komagataeibacter sp. nov. CGMCC 17276, which showed rapid cell growth and high BC-production ability, was isolated and classified into a novel species in the Komagataeibacter genus. Four BC synthase operons were annotated using whole-genome analysis, partially explaining the high BC yield of strain CGMCC 17276. Operons bcs Ⅱ and bcs Ⅲ showed high transcriptional levels under static and agitated culture conditions, indicating their importance in BC synthesis. Of the eight suitable carbon sources identified by whole-genome analysis, the highest BC production was achieved using glycerol as a single carbon source. Finally, waste glycerol was successfully used as an eco-friendly and sustainable strategy for BC production. This study provides valuable insights into the mechanism of BC synthesis, genetic structure of BC-producing strains, and industrialization of BC production using an eco-friendly and low-cost strategy.