Preparation and identification of monoclonal antibodies against Toxoplasma major surface antigen P30.

Objective: To develop and identify the monoclonal antibodies(McAb) against the major surface antigen P30 of Toxoplasma gondii, which may provide useful help for further study. Methods: BALB/c mice were immunized with the surface antigen of toxoplasma, and the spleen cells of immunized mice were fused with myeloma cell line SP2/0. After the cell fusion, positive hybridoma cell lines were screened by ELISA. Results: McAbs of E3 and G2 were identified to be against the major surface antigen P30 by SDS PAGE and Western Blot. These two clons of the McAbs were IgG1 subtypes by ELISA testing and the hybridoma cells could secrete higher titers of McAbs. The antigens recognized by McAbs by IFAT were located on the surface of tachyzoite. The McAbs had no cross reactions with antigens of Cryptosporidium、Pneumosystis caranii and Cysticercus cellucose. Conclusion: The hybridoma cell lines which can secrete high titers of anti P30 have been established.