Development and Validation of New Analytical Method for the Estimation of Tizanidine Hydrochloride in Bulk and in Formulation by UV Spectrophotometric Method

IUPAC Name of Tizanidine hydrochloride is 5-chloro-N-(4,5-dihydro-1H-imidazol-2-yl)-2,1,3- benzothiadiazol-4-amine. Tizanidine is a short-acting drug for the management of spasticity. Tizanidine is an agonist at α2-adrenergic receptor sites and presumably reduces spasticity by increasing presynaptic inhibition of motor neurons1.The effects of tizanidine are greatest on polysynaptic pathways. The overall effect of these actions is thought to reduce facilitation of spinal motor neurons.tizanidin comes in the form of a tablets and capsules. Present research work deals with UV spectrophotometric method for the estimation of tizanidin hydrochloride in pure form. For the estimation of tizanidine ,solvent system employed was distilled water and wave length of detection (λmax) was 268.5 nm. The linearity was obtained in the range 5-30 μg/ml, with a regression coefficient, R2=0.999. The LOD and LOQ were found to be 0.33μg/ml and 1.02820µg/ml respectively. The percentage recovery of Tizanidine hydrochloride sample was found with in the limit 96.48%- 105.76% Mean of SD 99.27±1.9216 (%RSD 1.9351,SE 0.7903), Mean of SD 98.51±1.8960(%RSD 1.9255,SE 0.7861). Obtained results showed that there is minimum intraday and interday variation. The developed method was validated and recovery studies were also carried out. Sample recovery using the above method was in good agreement with their respective labeled claims, thus suggesting the validity of the method and non-interference of formulation excipients in the estimation.