EFFECT OF AGING ON SPONTANEOUS AND INDUCED MOUSE TESTICULAR GERM CELL APOPTOSIS

The purpose of this study was to determine whether apoptotic cell death of mouse testicular germ cells varies with increased age, or with exposure to an acute systemic oxidative stress. Results show that the percent of seminiferous tubules with apoptotic cells, and the number of apoptotic cells/tubule cross section were not significantly altered with age. However, there were significantly more apoptotic metaphase spermatocytes at tubule stage XIV in 24-month-old mice than in 6-month-old mice. Oxidative stress significantly increased apoptotic metaphase spermatocytes in young mice, and severely reduced testicular apoptosis in old mice. Our results have potential clinical relevance to changes with increased age in human sperm aneuploidies.